Taurine Enhances Anticancer Activity of Cisplatin in Human Cervical Cancer Cells - Advances in Experimental Medicine and Biology - page 180

Fig. 19.2 Co-treatment of cisplatin with taurine increases apoptotic cells. Apoptotic cells were
imaged using DAPI as fluorescent probes. Cells were treated with 0 and 20 mM of taurine and
5 mM of cisplatin for 72 h. Arrows indicated the apoptotic cells

Fig. 19.3 Effects of cisplatin and taurine on p53 proteins. Protein expressions were measured using
western blotting. Cells were treated with cisplatin (5 mM) and taurine (5, 10, and 20 mM) for 72 h

19.3.5 In Co-treatment of Cisplatin with Taurine, Caspase-3

Was Increased, Whereas PARP Was Unchanged

Caspase-3 and PARP were detected by western blotting. Caspase-3 is an apoptosis-
regulating enzyme, and it leads to apoptosis. In single treatment of taurine, caspase-3
was increased in a dose-dependent manner, and combination treatment strongly acti-
vated caspase-3. PARP is assisting in the repair of single-strand DNA nicks, so it inhibits