Taurine Enhances Anticancer Activity of Cisplatin in Human Cervical Cancer Cells - Advances in Experimental Medicine and Biology - page 181

Fig. 19.4 Effects of cisplatin and taurine on caspase-6, caspase-7, and caspase-9 proteins. Protein
expressions were measured using western blotting. Cells were treated with cisplatin (5 m M) and
taurine (5, 10, and 20 mM) for 72 h

Fig. 19.5 Effects of cisplatin and taurine on apoptosis-related proteins, caspase-3, and PARP.
Protein expressions were measured using western blotting. Cells were treated with cisplatin (5 m M)
and taurine (5, 10, and 20 mM) for 72 h

apoptosis. The results indicated that single treatment of taurine or cisplatin did not affect
PARP expression. Similarly, expression of PARP was not increased in co-treatment of
cisplatin with taurine (Fig. 19.5 ).

19.4 Discussion

In the present study, we investigated the anticancer effects and mechanisms of sin-
gle treatment of taurine or co-treatment of cisplatin with taurine in human cervical
cancer HeLa cells in order to enhance anticancer effects and reduce side effects of
cisplatin.
After treatment of taurine for 48 h or 72 h, cell proliferation was significantly
decreased in a time- and dose-dependent manner. In previous studies, taurine is
known to promote cell proliferation in normal cell line (Chen et al. 1998 ; Lima and
Cubillos 1998 ; Hernández-Benítez et al. 2012 ). However, according to a recent