Biomedical Engineering Reference
In-Depth Information
Fig. 6. From top to bottom: patient homozygous for R160Q of the
SUOX
gene, a
normal individual; mother of the patient, polymerase chain reaction product mix of the
patient and the normal individual.
7.
The pressure of the system should be approx 104 psi at 0.05 mL/min flow of 50%
A/B and 1560 psi at 0.9 mL/min. The pressure should increase very slowly with
the increase in the number of injections. Any drastic increase signals severe clog-
ging of the column or other parts of the system. The column should be reverse
hot-washed and the whole system (with column removed) should be thoroughly
cleaned with 50% isopropanol.
8.
Use a 1.2-1.8% B/min gradient for the separation of larger fragments.
9.
Elution profiles of each single injection should be minimally 2 mV.
10.
To clean the column, run a Dwash for 15 min at 75°C at the end of each project.
11.
To extend the life span of the column when not in use, either keep the column
flushed with 50% A/B at 0.05 mL/min, or store the column capped in 75% ACN.
12.
When the flow of eluent is to be turned off for an extended period of time, i.e.,
3 d or more, remember to flush the separation cartridge with 75% ACN for
15 min, and remove it from the flowpath. Cap the ends of the cartridge tightly
with the supplied plugs, and replace with a PEEK union. Prior to returning the
cartridge to the system, flush the system with 75% ACN for 10 min; return the
