Biomedical Engineering Reference
In-Depth Information
cartridge to the system, and flush the cartridge with 75% ACN for 15 min.
Remember to equilibrate the cartridge with 50% A and 50% B at the appropri-
ate flow rates for 30 min.
13.
Standard procedures for operating a DHPLC instrument for mutation detection
(e.g., normal ranges for the critical parameters of the mutation standards) have
been published
(
14
)
.
14.
DHPLC can separate wild-type homoduplexes from mutant homoduplexes for
certain mutations (
Fig. 6
).
References
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20,
765-768.
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MECP2 gene in patients with infantile autism and Rett syndrome.
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37,
e41.
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matography (DHPLC) for prenatal diagnosis of genetic disease.
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22,
79-80.
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phy.
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Identification of a novel missense mutation (G149E) in glucose-6-phosphate
