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F i g u r e 5.3. Loss of polarization in hyperplastic HIV
germinal centers. Ki67 and IgD labeling of
normal tonsil (A, B), hyperplastic HIV
ÿ
(C, D), and HIV
(E, F ) lymph node sections. Labeling
was detected with 3,3
0
-diaminobenzidine chromogen. Sections were counterstained with Mayer's
hemalun and mounted. Original magni®cation (A±F): X25. LZ, Light zone; DZ, dark zone; MZ,
mantle zone.
Vyth-Dreese et al., who showed that CD80 expression was restricted to the
dark zone of GC ( Vyth Dreese et al., 1995), we found that CD80 staining
overlapped dark and light zones in tonsil sections (Fig. 5.4A). This may be due
to the use of di¨erent CD80 mAbs, BB1 or L307.4 versus B7.24 mAb. CD80
staining was weak or absent in all HIV
LNs but was observed in all HIV
ÿ
hyperplastic GC. We detected CD86 and CD80 on extrafollicular DC from
most sections. Cell suspensions from normal tonsils and from the LNs of three
HIV
patients were double-labeled with CD19 mAb and CD80 mAb or CD86
mAb. CD80 and CD86 expression was similar on CD19
B cells from tonsils
whereas CD80 expression was lost or decreased on the surface of CD19
B cells
from the three HIV
patients tested ( Fig. 5.5). In contrast, CD86 was similarly
expressed in these patients and in controls. As DC and FDC were scarce in cell
