B Cells in the Line of Sight of HIV-1 - Cellular Aspects of HIV Infection - page 81

Figure 5.4. Low level of CD80 in hyperplastic HIV lymph nodes. CD80 and CD86 labelings of
normal tonsil (A, B), hyperplastic HIV ÿ (C, D), and HIV (E, F ) lymph node sections. Original
magni®cation (A±F): X25. LZ, Light zone; DZ, dark zone; MZ, mantle zone.
suspensions, it was impossible to determine whether CD80 and CD86 expres-
sion was changed on these cells. There was no correlation between the magni-
tude of the decrease in CD80, the stage of the disease, antiretroviral therapy,
and the number of circulating CD4 T cells. Previous studies have reported
that peripheral HIV T cells express CD80 and CD86 antigens ( Wolthers et
al., 1996). In contrast, we did not detect these Ag on T cells from HIV LN
sections.
Studies in vitro have shown that CD80 is a late activation marker of APC
requiring CD86/CD28 and CD40/CD40L(CD154) interactions for its expres-