Chemistry Reference
In-Depth Information
Reagents +
solid support
Equilibration
by stirring
Filtration
Transferring
Detection
• Very high sensitivity
• Only one analyte
• High sample and reagents volumes
• Tedious and very time consuming
• Low sample throughput
Waste:
Solution +
beads
Sample
SPMS: Batch mode
Propulsion
system
Detection
Bead
discarding
Bead
injection
Waste:
Solution +
beads
Sample
injection
Additional
processes: on
line separation,
merging, etc
OS
• High sensitivity
• Higher reproducibility
• One or several analytes
• Low sample and reagents volumes
• High versatility
• Automation
• Higher sample throughput
• Solid support discarded after
measuring (only one measurement)
SPMS: Flow mode (Bead injection spectroscopy, BIS)
Detection
Sample
injection
Propulsion
system
Waste:
Solution
OS
• High sensitivity
• Higher reproducibility
• One or several analytes
• Low sample and reagents volumes
• High versatility
• Automation
• Higher sample throughput
• Re-use of sensing support (hundreds
of measurements)
Additional processes:
on line separation,
reagent merging, etc
SPMS: Flow mode (FIA, SIA, MC)
Figure 12.8
Schematic comparison of SPMS procedures from the GAC point of view.
