Biology Reference
In-Depth Information
0.4
0.3
0.2
0.1
0.0
10
20
30
40
50
60
Temperature (C)
FIGURE 9.30
Gel-to-liquid crystal transition of DPPC as followed by the steady state anisotropy of DPH.
Courtesy of Charles University in Prague, Faculty of Science.
where I is the emitted light intensity as measured through the vertical (V) or horizontal
(H) polarizer detectors. If no molecular motion occurs between the time of absorbance
and the time of emission, Anisotropy has a limiting value of 0.4. If rapid motion
occurs, the limiting value for Anisotropy is zero. From the decay of the Anisotropy
H
H
H
H
H
H
FIGURE 9.31
1,6-diphenylhexatriene (DPH).
