Silanization with APTES for Controlling the Interactions Between Stainless Steel and Biocomponents: Reality vs Expectation - Biomaterials: Physics and Chemistry - page 109

The O 1s peak was decomposed in three components (Landoulsi et al., 2008a). The first one,
at 529.7 eV, is due to inorganic oxygen in metal oxides (M-O) (NIST Database). The FWHM
of the two other components were arbitrarily imposed to be equal. The component at about
531.2 eV may be due to oxygen making a double bond with carbon (C=O including amide
and carboxyl group) and to oxygen of carboxylate. Contributions of metal hydroxides (M-
O-H) as well as oxygen bound to silicon [Si-O] in silane are overlapping with this
component (NIST Database; Genet et al., 2008). The last component, at 533.1 eV, is attributed
to oxygen making single bonds with carbon (C-O-H of alcohol and carboxyl, C-O-C of
ether and ester).

O 1s O 1s N 1s N 1s C 1s C 1s

nat nat

Mo 3p 3/2 Mo 3p 3/2 Mo 3p 3/2

53 8 538 53 6 536 53 4 534 53 2 532 53 0 530 52 8 528 52 6 526 40 4 404 404 40 2 402 402 40 0 400 400 39 8 398 398 39 6 396 396 39 4 394 394 39 2 392 392 39 0 390 390 29 4 294 29 2 292 29 0 290 28 8 288 28 6 286 28 4 284 28 2 282 28 0 280

Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV)

sil sil

53 8 538 53 6 536 53 4 534 53 2 532 53 0 530 52 8 528 52 6 526 40 4 404 40 2 402 40 0 400 39 8 398 39 6 396 39 4 394 39 2 392 39 0 390 29 4 294 29 2 292 29 0 290 28 8 288 28 6 286 28 4 284 28 2 282 28 0 280

Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV)

sil+Gox sil+Gox

538 534 530 526 538 534 530 526

53 8 538 53 6 536 53 4 534 53 2 532 53 0 530 52 8 528 52 6 526

404 400 394 392 404 400 394 392

40 4 404 40 2 402 40 0 400 39 8 398 39 6 396 39 4 394 39 2 392 39 0 390 29 4 294

294 290 286 282 294 290 286 282

29 2 292 29 0 290 28 8 288 28 6 286 28 4 284 28 2 282 28 0 280

Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV) Bin ding Energy (eV)

Binding Energy (eV) Binding Energy (eV)

Fig. 3. Decomposition of O 1s, N 1s and C 1s peaks recorded on: native stainless steel (nat),
silanized stainless steel (sil) and the same after adsorption of glucose oxidase (sil+Gox). The
N 1s peak is overlapped with a Mo 3p 3 / 2 contribution.