Biomedical Engineering Reference
In-Depth Information
d. AFM: increase of surface roughness, presence of nanostructures presumably due to
proteins or peptide.
Poly(dimethylsiloxane)
SSMCC
DNA
Vaidya & Norton, 2004
a. Surface plasma oxidation.
b. Vapor deposition, 30 min, 100 °C.
c. Hybridization of attached DNA. No blank.
d. XPS: elemental composition and peak shapes, demonstration of silane deposition.
Polystyrene
BS
3
, GMBS
IgG
North et al., 2010
a. Plasma treatment.
b. In ethanol+acetic acid; rinsing in methanol.
c. -
d. Fluorescence labeling: increase of amount of immobilized proteins by silanization +
linker step.
Cellulose
SMP
RGDC peptide
Bartouilh de Taillac et
al., 2004
a. Drying, outgassing.
b. In dry toluene under argon, 1.5 h; rinsing with dry toluene; outgassing.
c. Increase of osteoprogenitor cells attachment and proliferation. Blank = native.
d. XPS: qualitative consistency with expectations but probable Si contamination on final product.
BSA = bovine serum albumin
BS
3
= bis(sulfosuccinimidyl) suberate
CDI = 1,1'-carbonyldimidazole
DSC = N,N'-disuccinimidyl-carbonate
DSS = N,N'-disuccinimidyl-suberate
DS3 = N,N'-disulphosuccinimidyl-suberate
EDC = 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide.
ECM = extracellular matrix
EGF = epidermal growth factor
GA = glutaraldehyde
GMBS = 4-maleimidobutyric acid N-hydrosuccinimide ester
LbL = layer-by-layer
LC-SPDP = Succinimydil 6-[30-(2-pyridildithio)-propionamido]hexanoate
QCM = quartz crystal microbalance
RGDC = arginine - glycine - aspartic acid - cysteine
SMP = N-succinimidyl-3-maleimidopropionate
SSMCC = sulfosuccinimidyl-4-(N-maleimidomethyl)-cyclohexane-1-carboxylate
TNBS = 2,4,6-trinitrobenzenesulfonic acid
Table 1. Illustration of the use of APTES for retaining biomolecules on surfaces. The
meaning of a, b, c, and d is given in the upper box.
2. Materials and methods
2.1 Materials
Disks of 316L SS (0.74 cm
2
, from Outokumpu Stainless AB, ARC) were used. The bulk
chemical composition of 316L SS was given previously (Landoulsi et al., 2008a). All
chemicals (NaCl, Na
2
SO
4
, NaNO
3
NaHCO
3
, NaHPO
4
, Na
2
PO
4
) were provided by Prolabo
(VWR, France) and ensured 99% minimum purity. 3-aminopropyl(triethoxysilane) (APTES,
99%), glucose oxidase (Gox, EC 1.1.3.4, 47200 U.g
-1
) from
Aspergillus Niger
and
D
-glucose
