Biomedical Engineering Reference
In-Depth Information
for Ti-6Al-4V. Blank = native + enzyme, silanized + enzyme.
d. Wet chemical analysis of amino groups on silanized substrate only.
Magnesium
Ascorbic acid
BSA
Killian et al., 2010
a. Surface polishing; rinsing in water and ethanol.
b. In toluene, 24 h at 70 °C; rinsing in different solvents; sonication in ethanol.
c. -
d. XPS: elemental concentration, consistent evolution according to reaction steps. Tof-
SIMS: demonstration of silanization and protein retention.
Tantalum coating
DS3; DSS; DSC;
CDI
Collagen
Müller et al., 2005
a. Acid etching; rinsing with different solvents; drying in vacuum.
b. In boiling toluene under argon; rinsing in chloroform, methanol, and in water.
c. Increase of cell adhesion and proliferation (stem cell culture and subcutaneous
implantation). Blanks = native, silanized.
d. Wet chemical analysis: amino groups, collagen, consistent with expectation. AFM:
typical structure of fibrillar collagen.
SiO
2
, TiO
2
, Si
3
N
4
GA
Antigen
Kim et al., 2009b
a. Plasma treatment.
b. In ethanol/water (95/5, v/v), rinsing with ethanol ; curing 15 min at 120 °C.
c. Antigen/antibody test. Influence of plasma exposure time.
d. Water contact angle and XPS on cleaned substrate, influence of plasma exposure time.
Silicon dioxide
SMP
RGD peptide
Davis et al., 2002
a. Oxidation and hydroxylation; rinsing with different solvents.
b. In dry toluene, 120 °C, 3 h; sonication in various organic solvents and water.
c. Increase of fibroblast proliferation. Blanks = no RGD.
d. XPS: elemental concentration, consistent evolution according to reaction steps. AFM:
small clumps after APTES step ; larger clumps after APTES+SMP step.
Silicon dioxide
GA
Glucose oxidase
Libertino et al., 2008
a. Oxidation in a solution of ammonia and hydrogen peroxide.
b. Vapor deposition; curing 40 min at 80 °C under vacuum.
c. Slight decrease of the enzyme activity when the surface is not oxidized (a). No blank.
d. AFM: consistent evolution of surface roughness according to reaction steps. XPS:
elemental concentration, consistent evolution according to reaction steps.
Silicon dioxide LC-SPDP Tagged Kcoil peptides Boucher et al., 2009
a. Piranha treatment, 10 min at 100°C; rinsing in water; drying in air; storing in vacuum.
b. In anhydrous toluene, 3 h; curing 1.5 h at 120°C; sonication in freshly distilled toluene.
c. Improvement of binding efficiency (amount, affinity
)
of Ecoil-tagged EGF. Blank =
silanized + LC-SPDP with blocked termination.
d. Ellipsometry and water contact angle: consistent evolution according to reaction steps.
Glass
none
Proteins from ECM,
RGD peptide
Siperko et al., 2006
a. Cleaning in different solvent.
b. In anhydrous ethanol + acetic acid, followed by addition of ultrapure water, 5 min;
curing 15 min at 120°C.
c. Improvement of osteoblast adhesion and growth. Blank = silanized.
