Chemistry Reference
In-Depth Information
Fig. 6.49
Representation of the DDMC/PTX complex. Simplified structures are shown. The
DDMC/PTX complex (~270 nm) consists of more than 8.1
10
3
DDMC molecules and
6.7
10
6
paclitaxel molecules
approximately 0.2
m in size . Transparent dextran-matrix copolymers (DMC) have
recently been prepared for HCL and IOL applications that consist of the PEC
between cationic dextran and unsaturated acids with compounds of vinyl. The
PEC is expected to be a biocompatible material having an O
2
transport ability.
We have evaluated the biocompatibility of DMC for a human tear by examining the
drying pattern of NaCl salt crystals from a 0.2 % solution of human
μ
-globulin
dissolved in physiological saline, as proposed by Sole. We think that the DMC has
good biocompatibilities, not only because of its structure containing a hydrophili-
c-hydrophobic microseparated domain owing to a PEC, but also because of an
electric charge distribution that is localized by the polyanion of excess. Moreover,
we have clarified the role of dextran in the affinity of DMC to tear liquid.
A stable latex of DEAE-dextran-MMA graft copolymer (DDMC) was developed
for non-viral gene delivery vectors that can be autoclaved at 121
C for 15 min.
Transfection activity was determined using the X-gal staining method, and a higher
value of 5-10 times or more was confirmed for DDMC samples than for the starting
DEAE-dextran hydrochloride. DDMC has been also confirmed as having a high
protection facility against DNase degradation. The DDMC, having an amphiphilic
domain, forms a core-shell polymer micelle that should become a stable latex with
a hydrophilic-hydrophobic microseparated domain. The complex of DDMC and
DNA can be formed initially on the stable spherical structure of the amphiphilic
microseparated domain of DDMC and has a good affinity to the membrane of the
cell for endocytosis. The shift in the IR absorption spectrum of the complexes
between DNA and DDMC to a high energy direction at around 3,450 cm
1
may
γ
