Use of Dodecahedron “VLPs” as an Alternative to the Whole Adenovirus - Adenovirus: Methods and Protocols - page 68

Fig. 4 Labeled dodecahedron in HeLa cells. ( a ) Fixed cell. FITC-labeled Dd was
incubated for 1 h. at 37 °C with HeLa cells prior to methanol fi xation. Nuclei were
counterstained by propidium iodide. Z-series was acquired from bottom to top in
both green and red channels with a 0.2 m step interval. Left panel “Bottom

slice”; right panel “superposition of all Z slices”. Note that signal is acquired in
gray scale enabling the pseudocoloring of the green FITC in blue. ( b ) Living-cell
experiment using HeLa cells expressing GFP-microtubules. Cy3-Dd addition
decorates this cell captured in a Z-series. The adjacent panels show XZ and YZ
slabs, while medium picture shows one medium XY slice of the Z-series

μ

refractory index between background and reference cells.
Ideally, all the samples should be dialyzed in running buffer
prior to injection to minimize the shift as shown in Fig. 3 ,
adapted from [ 12 ] with permission.