Biology Reference
In-Depth Information
11. Scatchard analysis can be done for complicated analyte/ligand
interaction. For this reason, a maximum number of curves
must reach a plateau. Maximum binding values at the plateau
(in RU) are then plotted along the “
x
axis” while Bound/Free
(RU/M “
M
= Molarity”) are plotted along the “
y
axis”. The
slope “
s
” is then calculated giving a negative number in (1/
M
).
K
D
= −(1/
s
) and is expressed in M.
Acknowledgments
I acknowledge the French “CNRS” for support throughout the
dodecahedron story and all the coauthors of publications in this
fi eld. I am grateful to Lucy Freeman for English read-through.
References
1. Zeltins A (2012) Construction and character-
ization of virus-like particles: a review. Mol
Biotechnol 53(1):92-107
2. Fender P, Ruigrok RW, Gout E, Buffet S,
Chroboczek J (1997) Adenovirus dodecahe-
dron, a new vector for human gene transfer.
Nat Biotechnol 15:52-56
3. Schoehn G, Fender P, Chroboczek J, Hewat
EA (1996) Adenovirus 3 penton dodecahe-
dron exhibits structural changes of the base on
fi bre binding. EMBO J 15:6841-6846
4. Fuschiotti P, Schoehn G, Fender P, Fabry CM,
Hewat EA, Chroboczek J et al (2006) Structure
of the dodecahedral penton particle from human
adenovirus type 3. J Mol Biol 356:510-520
5. Vives RR, Lortat-Jacob H, Chroboczek J,
Fender P (2004) Heparan sulfate proteoglycan
mediates the selective attachment and internal-
ization of serotype 3 human adenovirus
dodecahedron. Virology 321:332-340
6. Gout E, Schoehn G, Fenel D, Lortat-Jacob H,
Fender P (2010) The adenovirus type 3
dodecahedron's RGD loop comprises an
HSPG binding site that infl uences integrin
binding. J Biomed Biotechnol 2010:541939
7. Wang H, Li ZY, Liu Y, Persson J, Beyer I,
Moller T et al (2011) Desmoglein 2 is a recep-
tor for adenovirus serotypes 3, 7, 11 and 14.
Nat Med 17:96-104
8. Fender P, Schoehn G, Foucaud-Gamen J, Gout E,
Garcel A, Drouet E et al (2003) Adenovirus
dodecahedron allows large multimeric protein
transduction in human cells. J Virol 77:
4960-4964
9. Garcel A, Gout E, Timmins J, Chroboczek J,
Fender P (2006) Protein transduction into
human cells by adenovirus dodecahedron using
WW domains as universal adaptors. J Gene
Med 8:524-531
10. Villegas-Mendez A, Garin MI, Pineda-Molina
E, Veratti E, Bueren JA, Fender P et al (2010)
In vivo delivery of antigens by adenovirus
dodecahedron induces cellular and humoral
immune responses to elicit antitumor immu-
nity. Mol Ther 18:1046-1053
11. Villegas-Mendez A, Fender P, Garin MI, Rothe
R, Liguori L, Marques B et al (2012) Functional
characterisation of the WW minimal domain
for delivering therapeutic proteins by adenovi-
rus dodecahedron. PLoS One 7:e45416
12. Wang H, Li Z, Yumul R, Lara S, Hemminki A,
Fender P et al (2011) Multimerization of ade-
novirus serotype 3 fi ber knob domains is
required for effi cient binding of virus to des-
moglein 2 and subsequent opening of epithelial
junctions. J Virol 85:6390-6402
13. Szolajska E, Burmeister WP, Zochowska M,
Nerlo B, Andreev I, Schoehn G et al (2012)
The structural basis for the integrity of adeno-
virus ad3 dodecahedron. PLoS One 7:e46075
14. Fender P, Hall K, Schoehn G, Blair GE (2012)
Impact of human adenovirus type 3 dodecahe-
dron on host cells and its potential role in viral
infection. J Virol 86:5380-5385
