Biology Reference
In-Depth Information
Table 1
Standard scheme of the medium (DMEM + 2 % FBS) and virus volumes for the serial dilutions of viral stock titration
24-Well plate
Dil #:
1
2
3
4
5
6
7
8
9
10
11
12
13
14
Medium (ml)
2
2
1.8
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
0.5
+
2
μ
l virus
2
μ
l #1
0.2 ml
#2
0.5 ml
#3
0.5 ml
#4
0.5 ml
#5
0.5 ml #6
0.5 ml #7
0.5 ml #8
0.5 ml #9
0.5 ml #10 0.5 ml #11 0.5 ml
#12
0.5 ml #13
Relative
dilution factor
1/1,000 1/1,000 1/10 1/2
1/2
1/2
1/2
1/2
1/2
1/2
1/2
1/2
1/2
1/2
Absolute
dilution factor
1/10
3
1/10
6
1/10
7
1/2 · 10
7
1/4 · 10
7
1/8 · 10
7
1/1.6 · 10
8
1/3.2 · 10
8
1/6.4 · 10
8
1/1.3 · 10
9
1/2.6 · 10
9
1/5.2 · 10
9
1/10
10
1/2 · 10
10
