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In-Depth Information
14. When infecting, start adding the most diluted point and
use the same tip while infecting with the same virus. Try to
release the 100
L of infection medium/well without disturb-
ing the monolayer (particularly for HEK293 cells). Try not to
make bubbles.
15. Time must be chosen depending on rate of replication of the
adenovirus in the cell line used in order to avoid secondary
infections.
16. For most hybridomas 1/5 dilution from supernatant is recom-
mended. If using a purifi ed anti-adenovirus or anti-hexon Ab,
1/500 dilution can be initially tested.
μ
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