Rapid and High-Throughput N -Glycomic Analysis of Plant Glycoproteins - Plant Proteomics: Methods and Protocols - page 622

Table 1
N -glycome in rice AmyI-1

m/z a [M+H] + Δ b Estimated N -glycan structures c pmol/25 μg protein

831.13 −0.33 Internal standard (GN4) 50.00

895.15 −0.19 Hex 2 HexNAc 2 dHex 1 0.01

911.16 −0.17 Hex 3 HexNAc 2 0.01

936.21 −0.16 Hex 1 HexNAc 3 dHex 1 0.02

1,027.21 −0.17 Hex 2 HexNAc 2 dHex 1 Pent 1 0.03

1,043.19 −0.18 Hex 3 HexNAc 2 Pent 1 10.01

1,057.22 −0.18 Hex 3 HexNAc 2 dHex 1 0.07

1,114.24 −0.18 Hex 3 HexNAc 3 0.06

1,189.26 −0.18 Hex 3 HexNAc 2 dHex 1 Pent 1 0.54

1,246.28 −0.17 Hex 3 HexNAc 3 Pent 1 23.02

1,260.31 −0.16 Hex 3 HexNAc 3 dHex 1 0.25

1,276.37 −0.10 Hex 4 HexNAc 3 0.01

1,351.35 −0.14 Hex 4 HexNAc 2 dHex 1 Pent 1 0.02

1,392.35 −0.16 Hex 3 HexNAc 3 dHex 1 Pent 1 0.76

1,397.35 −0.14 Hex 6 HexNAc 2 0.01

1,406.46 −0.07 Hex 3 HexNAc 3 dHex 2 0.01

1,408.36 −0.15 Hex 4 HexNAc 3 Pent 1 0.16

1,449.37 −0.16 Hex 3 HexNAc 4 Pent 1 56.02

1,463.40 −0.15 Hex 3 HexNAc 4 dHex 1 1.18

1,559.42 −0.13 Hex 7 HexNAc 2 0.00

1,595.45 −0.14 Hex 3 HexNAc 4 dHex 1 Pent 1 2.26

1,609.47 −0.14 Hex 3 HexNAc 4 dHex 2 0.01

1,652.50 −0.12 Hex 3 HexNAc 5 Pent 1 0.02

1,682.48 −0.15 Hex 4 HexNAc 5 0.21

1,757.52 −0.13 Hex 4 HexNAc 4 dHex 1 Pent 1 0.75

1,771.59 −0.07 Hex 4 HexNAc 4 dHex 2 0.01

1,773.57 −0.08 Hex 5 HexNAc 4 Pent 1 0.01

1,800.49 −0.19 Hex 3 HexNAc 3 dHex 2 Pent 3 0.03

1,962.57 −0.16 Hex 3 HexNAc 3 dHex 4 Pent 2 0.01

a m /z of free glycan [M+H] +
b Difference from the theoretical mass value
c Structural annotation was achieved using GlycoMod tool online database. Hex, hexose; HexNAc, N -acetyl
hexosamine; dHex, deoxyhexose; Pent, pentose