Biology Reference
In-Depth Information
Table 1
Commercially available glycan-binding lectins for the enrichment of glycoproteins
Lectin type
Name
Source
Affi nity
Mannose binding
lectins
Con A (concanavalin A)
Canavalia
ensiformis
High-mannose, hybrid and
biantennary complex type
N
-glycans [
30
-
33
]
LCH (Lentil lectin)
Lens culinaris
Fucosylated core region of
bi- and triantennary complex
type
N
-glycans [
34
]
GNA (Snowdrop lectin)
Galanthus
nivalis
α
-1,3 and
-1,6 link high
mannose structure [
35
]
α
Fucose binding
lectins
UEA (
Ulex europaeus
agglutinin)
Ulex
europaeus
Fuc
α
1-2Gal-R [
36
]
α
β
α
AAL (
Aleuria aurantia
)
Aleuria
aurantia
Fuc
1-2Gal
1-4(Fuc
13/4)
β
Gal
1-4GlcNAc; R
2
-
GlcNAc
β
α
1-4(Fuc
1 6)
GlcNAc-R
1
[
37
]
Galactose/
N-
acetylgalactosamine
binding lectins
RCA (
Ricinus communis
Agglutinin)
Ricinus
communis
Gal
β
1-4GlcNAc
β
1-R [
38
,
39
]
PNA (Peanut Agglutinin)
Arachis
hypogaea
Gal
1-Ser/Thr
(T-Antigen) [
40
-
42
]
β
1-3GalNAc
α
AIL (Jacalin)
Artocarpus
integrifolia
(Sia)Gal
1-Ser/
Thr (T-Antigen) [
43
]
β
1-3GalNAc
α
VVL (Hairy vetch lectin)
Vicia villosa
GalNAc
-Ser/Thr
(Tn-Antigen) [
44
,
45
]
α
Sialic acid/
N-
acetylglucosamine
binding lectins
WGA (Wheat Germ
agglutinin)
Triticum
vulgaris
GlcNAc
β
1-4GlcNAc
β
1-
4GlcNAc, Neu5Ac (sialic
acid) [
46
,
47
]
SNA (Elderberry lectin)
Sambucus
nigra
Neu5Ac
α
2-6Gal(NAc)-R [
48
]
MAL (
Maackia
amurensis
lectin)
Maackia
amurensis
Neu5Ac/Gc
α
2-3Gal
β
1-
4GlcNA
cβ
1-R [
49
]
2
Materials
Note: mention of specifi c companies or pieces of equipment does
not represent an endorsement by the authors.
Several lectins coupled to Sepharose beads, magnetic beads [
22
,
23
]
or to beads packed into chromatography columns/cartridges are
commercially available (e.g., Qiagen) to facilitate the enrichment
of a diverse population of glycoproteins (Table
1
). Of these,
Concanavalin A (Con A) is the most frequently used. It is possible to
work with several lectins sequentially [
24
,
25
], in parallel [
8
,
14
,
26
]
2.1 Lectin Resins
