Plant Cell Lines in Cell Morphogenesis Research - Plant Cell Morphogenesis: Methods and Protocols - page 219

unchanged (ca. 30-40 m for VBI-0 and

BY-2, respectively), but their length increases 3-5 times. The
elongated stationary cells/cell couples forming the inocula
start to divide after short lag-phase (1-2 days) in the process
of stepwise septation. Consequently, daughter cells become
almost isodiametric. Based on the culture conditions, typi-
cally the number of cells in fi les can reach 8-12 but some-
times up to 24. The shortest length of the mitotic cycle
oscillates between 12 and 16 h. New cell elongation starts by
the end of exponential phase. Later on, the fi les spontane-
ously disintegrate again.

μ m and 20-30 μ

Acknowledgments

The authors acknowledge support for their work by the Grant
Agency of the Czech Republic (projects P305/11/0797 and
P305/11/2476), Ministry of Education, Youth and Sport of
the Czech Republic (project MSM00216208858), and Charles
University in Prague (project SVV 265203/2012).

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