Chemistry Reference
In-Depth Information
Dilution
MO/DOPE-PEG
2000
/OA = 83/2/15
MO/DOPE-PEG
2000
/EPA = 83/2/15
20
15
15
5 µL in 2 mL
DMEM
10
10
5
5
0
0
1
10
100
1000
10000
1
10
100
1000
10000
Size (d.nm)
Size (d.nm)
20
15
15
10 µL in 2 mL
DMEM
10
10
5
5
0
0
1
10
100
1000
10000
1
10
100
1000
10000
Size (d.nm)
Size (d.nm)
15
15
50 µL in 2 mL
DMEM
10
10
5
5
0
0
1
10
100
1000
10000
1
10
100
1000
10000
Size (d.nm)
Size (d.nm)
15
15
100 µL in 2 mL
DMEM
10
10
5
5
0
0
1
10
100
1000
10000
1
10
100
1000
10000
Size (d.nm)
Size (d.nm)
15
15
200 µL in 2.2
mL DMEM
10
10
5
5
0
0
1
10
100
1000
10000
1
10
100
1000
10000
Size (d.nm)
Size (d.nm)
Figure 11.8
Stability of NP size (expressed in vol %) distributions in lipid dis-
persions MO/DOPE - PEG
2000
/OA (83/2/15 mol %) and MO/DOPE - PEG
2000
/EPA
(83/2/15 mol %) determined by QELS upon sample dilutions in DMEM culture
medium.
are presented in Figure 11.9. The scattering curves of the aqueous disper-
sions of MO/DOPE - PEG
2000
/EPA (83/2/15 mol %) and MO/DOPE - PEG
2000
(98/2 mol %) self-assembled NP systems show similar structural features and
trends. It can be concluded that the incorporation of 15 mol % of a bioactive
component EPA does not dramatically infl uence the internal organization of
the nanocarriers. The polyunsaturated fatty acid appears to mix homoge-
neously with the main monoglyceride component (MO) in the generated
multicompartment lipid particles.
11.4.3 In Vitro Assays with Human Neuroblastoma SH-SY5Y
Cell Line
11.4.3.1 TrkB Receptor Expression in Differentiated Human Neuro-
blastoma Cells
The expression of the neurotrophin receptor TrkB,
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