Chemistry Reference
In-Depth Information
Dilution 1/10
Dilution 1/1000
15
15
MO/
DOPE-
PEG
2000
=
98/2
10
10
5
5
0
0
1
10
100
1000 10000
1
10
100
1000 10000
Size (d.nm)
Size (d.nm)
Figure 11.5
Stability of NP size (expressed in vol %) distribution in the lipid disper-
sion MO/DOPE-PEG
2000
(98/2 mol/mol) determined by QELS upon dilutions of 1/10
and 1/1000 in buffer.
20
15
10
5
0
1/6
(a)
DO/[total lipid] (L/mol)
350
300
250
200
150
100
50
0
0
1
10
100
Size (d.nm)
1000
10000
(b)
1/6
1/8
1/10
1/12
1/14
1/16
1/18
1/20
1/22
1/24
1/8
15
10
5
0
1
10
100
1000
10000
Size (d.nm)
1/10
20
15
10
5
0
5E-11
1E-10
1,5E-10
1
10
100
1000
10000
1/
λ
4
(1/nm
4
)
Size (d.nm)
20
15
10
5
0
1/12
1
10
100
1000
10000
Size (d.nm)
15
10
5
0
1/14
1
10
100
1000
10000
Size (d.nm)
Figure 11.6
Effect of dilution with buffer on the volume distribution of nanoobject
sizes in the MO/DOPE-PEG
2000
/OA (88/2/10 mol %) dispersed sample established by
(a) QELS and (b) by optical density (OD) measurements.
For the example of charged multicompartment lipid nanocarriers, QELS
and OD measurements were performed with the MO/DOPE-PEG
2000
/OA
(88/2/10 mol %) sample on the fourth day after its preparation in order to
study its NP size distribution stability over time and upon dilution (Fig. 11.6).
The two types of NP populations remain in coexistence in the aqueous medium
upon dilution. Aggregates of particles begin to grow at dilutions beyond 1/10.
Indeed, the dilution can modify the distribution of DOPE-PEG
2000
between
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