Multicompartment Lipid Nanocarriers for Targeting of Cells Expressing Brain Receptors - Self-Assembled Supramolecular Architectures: Lyotropic Liquid Crystals - page 333

N° of days J0 J2

25
20
15
10
5
0

25
20
15
10
5
0

MO/
DOPE-
PEG 2000 =
98/2

1 10 100

Size (d.nm)

1000 10000

1 10 100 1000 10000

Size (d.nm)

15
10
5
0

20
15
10
5
0

MO/
DOPE-
PEG 2000 /OA
= 93/2/5

1 10 100 1000 10000 1 10 100 1000 10000

Size (d.nm)

Size (d.nm)

15
10
5
0

15
10
5
0

MO/
DOPE-
PEG 2000 /OA
= 88/2/10

1 10 100 1000 10000

1 10 100 1000 10000

Size (d.nm)

Size (d.nm)

20
15
10
5
0

20
15
10
5
0

MO/
DOPE-
PEG 2000 /OA
= 83/2/15

1 10 100 1000 10000 1 10 100 1000 10000

Size (d.nm) Size (d.nm)

20
15
10
5
0

20
15
10
5
0

MO/
DOPE-
PEG 2000 /OA
= 78/2/20

1 10 100 1000 10000

1 10 100 1000 10000

Size (d.nm) Size (d.nm)

25
20
15
10
5
0

15
10
5
0

MO/
DOPE-
PEG 2000 /OA
= 69/1/30

1 10 100 1000 10000

1 10 100 1000 10000

Size (d.nm)

Size (d.nm)

Figure 11.4 Stability over time of NP size distributions in lipid dispersions MO/
DOPE- PEG 2000 /OA containing increasing molar fractions of oleic acid (0, 5, 10, 15, 20,
30 mol % OA). J0 denotes the day of the NP preparation, whereas J2 denotes 2 days
of NP storage at room temperature.

dilution beyond 1/30 should provoke the dissociation of eventually presenting
micelles in the system as the cmc of DOPE-PEG 2000 is about 2

1 0 − 5 M .
Micelles could not be measured by QELS upon dilution to lower DOPE-
PEG 2000 concentrations. Based on the determined characteristic sizes, the
results in Figure 11.5 should be attributed to the formation of PEGylated
multicompartment lipid particles. The aggregation of the MO/DOPE-PEG 2000
(98/2 mol/mol) NPs into larger liquid crystalline objects upon dilution is typical
for cubosome and spongosome organizations.

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