Biomedical Engineering Reference
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from ben saalga, a traditional pearl millet fermented food from Burkina Faso) pro-
duced strong inhibitory activity in malted millet fl our, decreasing the survival of B.
cereus , E. coli O157:H7 and S. enterica (Sánchez Valenzuela et al. 2008 ). The
authors of this study suggested that this strain could be applied as a starter culture
to improve the microbiological safety of cereal gruels.
Bacteriocin-producing strains can be isolated from raw as well as malted barley.
Fermented worts containing bacteriocins could be used to prevent beer spoilage
LAB (Vaughan et al. 2005 ). Bacteriocin production may be a desirable trait for wort
bioacidifying LAB starter cultures, enhancing the implantation and proliferation of
such strains over spoiling LAB. Development of yeast starter cultures genetically
modifi ed to produce bacteriocins has also been suggested, and heterologous produc-
tion of bacteriocins such as pediocins, leucocins, plantaricins and enterocins by
yeasts has been reported (Schoeman et al. 1999 ; Du Toit and Pretorius 2000 ; Van
Reenen et al. 2002 ; Gutiérrez et al. 2005 ; Sánchez et al. 2008 ; Basanta et al. 2009 ).
The bactericidal yeast strains could be used as starters or protective cultures in the
fermentations of brewing, wine and baking processes as biological control agents to
inhibit growth of spoilage bacteria.
Several bacteriocinogenic LAB strains have been isolated from wine and wine-
yards, including species of L. plantarum , O. oeni and P. pentosaceus (Lonvaud-
Funel and Joyeux 1993 ; Strasser de Saad and Manca de Nadra 1993 ; Navarro et al.
2000 ; Rojo-Bezares et al. 2007 ; Knoll et al. 2008 ; Yanagida et al. 2008 ). Selected
bacteriocin-producing strains could be useful against undesired LAB (such as hista-
mine producers or spoiler) in vinifi cation, and also for proper control of the wine
malolactic fermentation (Yurdugül and Bozoglu 2002 ) .
References
Abriouel H, Lucas R, Ben Omar N et al (2010) Potential applications of the cyclic peptide entero-
cin AS-48 in the preservation of vegetable foods and beverages. Probiot Antimicrob Prot
2:77-89
Allende A, Martínez B, Selma V et al (2007) Growth and bacteriocin production by lactic acid
bacteria in vegetable broth and their effectiveness at reducing Listeria monocytogenes in vitro
and in fresh-cut lettuce. Food Microbiol 24:759-766
Ananou S, Gálvez A, Martínez-Bueno M et al (2005) Synergistic effect of enterocin AS-48 in
combination with outer membrane permeabilizing treatments against Escherichia coli
O157:H7. J Appl Microbiol 99:1364-1372
Atrih A, Rekhif N et al (1993) Detection and characterization of a bacteriocin produced by
Lactobacillus plantarum C19. Can J Microbiol 39:1173-1179
Bari ML, Ukuku DO, Kawasaki T et al (2005) Combined effi cacy of nisin and pediocin with
sodium lactate, citric acid, phytic acid, and potassium sorbate and EDTA in reducing the
Listeria monocytogenes population of inoculated fresh-cut produce. J Food Prot
68:1381-1387
Bartowsky EJ (2009) Bacterial spoilage of wine and approaches to minimize it. Lett Appl
Microbiol 48:149-156
Basanta A, Herranz C, Gutiérrez J et al (2009) Development of bacteriocinogenic strains of
Saccharomyces cerevisiae heterologously expressing and secreting the leaderless enterocin
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