Biomedical Engineering Reference
In-Depth Information
Highly pure insulin
Fermentation
Cell recovery,
homogenization,
refolding of proinsulin
RP-HPLC
Crude proinsulin
Purified insulin
Primary purification,
e.g. crystallization
Ion exchange
and gel filtration
Enzymatic
proteolysis
Partially purified
proinsulin
Crude insulin
Figure 11.3
A likely purifi cation scheme for human insulin
prb
. A fi nal RP-HPLC polishing step yields a highly
pure product. Refer to text for details
Figure 11.4
Process-scale HPLC column. Photograph courtesy of NovaSep Ltd, http://www.novasep.com
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