Biology Reference
In-Depth Information
BOX 6.1 Synthetic Genetic Array (SGA)
e
Continued
CAN1
can1
Δ::
STE2pr-Sp_his5
MAT
α
query
MAT
a
xxx
Δ
LYP1
lyp1
Δ
Mating
Wild-type alleles
Deletion mutations
MAT
α/
a
Sporulation
MAT
a haploid selection ( )
Single mutant selection ( )
Double mutant selection ( )
lethal interactions are rich in functional information often
connecting genes with related biological functions and
uncovering novel functional relationships
[3]
. Furthermore,
this preliminary network highlighted the extensive genetic
complexity encoded within the genome of a single
unicellular organism. Indeed, estimates based on this small
sampling of the yeast genetic network (~3%) suggested that
a non-essential gene has, on average, approximately 30
interaction partners and, after considering the potential
contribution of essential gene interactions, the complete S.
cerevisiae was predicted to comprise ~200 000 synthetic
sick or lethal interactions
[3,22]
. This finding implies that
the potential for generating synthetic genetic interactions
should increase with gene number, and the genomic load of
synthetic effects may be even higher in more complex
multicellular organisms, including humans
[3,7,23]
.
