Biomedical Engineering Reference
In-Depth Information
reaction is a possible explanation of these promising results. We also attempted to prepare
cell-enclosing microcapsules of more than 400 μm in diameter using the same process. The
time necessary for obtaining gelated capsules increased with the increasing diameter of the
droplet. This finding shows that diffusion of H
2
O
2
from liquid paraffin is limited. Sufficient
oxygenation is needed for survival of cells, thus a shorter period of suspension in liquid
paraffin is preferable. These findings indicate that the peroxidase-catalyzed cell-encapsulation
process using liquid paraffin containing dissolved H
2
O
2
is more suitable for the production of
the microcapsules of about 100 μm in diameter.
Figure 12. (a) Micrographs of CRFK cells cultured on cell culture dishes of 60 mm in diameter at 4 and
20 hours after seeding. Recovered: cells seeded after being recovered from Alg-Ph capsules by
degradation using alginate lyase. Non-treated: cells seeded using a general subculture protocol. Cells
were seeded at 3.0 × 10
5
viable cells/well. (b) Proliferation profiles of CRFK cells on tissue culture
dishes. Columns indicate mean densities of (black) recovered cells and (white) non-treated cells. Error
bars: SDs (Reproduced with permission, from Sakai S et al. Biomacromolecules [39] @ 2007 American
Chemical Society).
