On the Influence of Melafen - Plant Growth Regulator - Progress in Organic and Physical Chemistry: Structures and Mechanisms - page 248

But Melafen has a bidirectional effect to the EAC cells (Table 1). We founded that
at super low concentrations Melafen (10 -12 and 10 -13 Ɇ) stimulated the signal trans-
duction, increasing the ɋɚ 2+ releasing from intracellular ɋɚ 2+ store (the ¿ rst peak).
But under the bigger concentrations the Melafen actions changed its vector and the
depressing of the overall cell responses were began. At the case, when the Melafen
concentrations were increased, the amplitudes of ¿ rst and second extremes were de-
creased. The second cell response - the ɋɚ 2+ entering through the plasma membrane
(second peak) was not activated by Melafen (under the anywhere concentrations), and
it shown the bigger sensitivity to the Melafen action. It was inhibited by the Melafen at
the smaller concentrations. Thus, Melafen 10 -7 Ɇ decreased by 50% the ¿ rst extreme
and by 70% - the second extreme. Melafen 10 -4 Ɇ really eliminated the overall cell re-
sponse fully; it inhibited the purine-dependent ɋɚ 2+ transduction - both peaks. Hence,
the carcinoma cells that characterized by uncontrolled cell division, can be depressed
by such doses of Melafen, which are harmless for erythrocytes that we obtain earlier
[16].

TABLE 1 The influences of Melafen to the first (A) and second (B) cellular answer of EAC
cells at the wide concentrations diapason.

The melafen influences to EAC cell bimodal responses (1 peak and 2 peak) under the ATP adding

Sample +melafen 1 peac (rel.un.) D (%) 1 peac (rel.un.) D (%)

control 27,5 ± 0,1 - 20 ± 0,1 -

+10 -13 M 35 ± 0,1 +27 ± 0,01 19 ± 0,1 -5 ± 0,01

+ 10 -12 M 33 ± 0,1 +20 ± 0,01 19 ± 0,1 -5 ± 0,01

+10 -11 M 27,5 ± 0,1 0 13 ± 0,1 -35 ± 0,01

+10 -10 M 25 ± 0,1 0 13 ± 0,1 -35 ± 0,01

+10 -9 M 22 ± 0,1 -20 ± 0,01 10 ± 0,1 -50 ± 0,01

+10 -8 M 16 ± 0,01 7 ± 0,1 -65 ± 0,01

+10 -7 M 13 ± 0,1 -53 ± 0,01 6 ± 0,1 -70 ± 0,01

+10 -6 M 12 ± 0,1 -56 ± 0,01 3 ± 0,1 -85 ± 0,01

+10 -5 M 11 ± 0,1 -60 ± 0,01 2 ± 0,1 -85 ± 0,01

+10 -4 M 7 ± 0,1 -74,5 ± 0,01 0 -100

+10 -3 M 0 -100 0 -100

Thus, Melafen inÀ uence on two targets surfaces of cells simultaneously on puri-
noreceptors PY2 and on CRAC, considerably reducing their activity in plants growth
stimulated doses. Really, Melafen changes the functions of surface receptors and in-
tracellular signal transduction.