From the Producer Microorganisms to the Lasso Scaffold - Lasso Peptides: Bacterial Strategies to Make and Maintain Bioactive Entangled Scaffolds - page 19

Table 2.5 (continued)

Peptide Peptide

length (aa)

Peptide net
charge a

Method for
analysis of 3D
structure

Ring size
(aa (Nb of
atoms))

Ring forming
residue

Lasso stabilization
plugs b

C-terminal
tail
size (aa)

References

BI-32169 19 12− NMR

X-ray

9 (25) Asp9 1 S-S bridge

Cys6-Cys19/
Trp13 / Trp17

10 (Knappe et al. 2010 ; Nar

et al. 2010 )

Proteobacteria
Astexin 1(23) d
Astexin 1(19)
Astexin 2
Astexin 3

23
19
24
24

4−
3−
2−
2−

NMR
N.E./Char

9 (28)
9 (28)
9 (28)
9 (28)

Asp9
Asp9
Asp9
Asp9

Tyr14/ Phe15
Tyr14/ Phe15
Tyr15/ Trp16

14
10
15
15

(Maksimov et al. 2012 ; Zim-
mermann et al. 2013 )

Burhizin 17 4− N.E./Char 8 (26) Glu8 n.i. 9 (Hegemann et al. 2013b )

Caulonodin I d
Caulonodin II d
Caulonodin III d

21/17
21/17
21/17

2−/2−
3−/1−
2−/1−

N.E./Char
N.E./Char
N.E./Char

8 (26)
8 (26)
8 (26)

Glu8
Glu8
Glu8

n.i. 13/9

13/9
13/9

(Hegemann et al. 2013b )

Capistruin 19 1+ NMR 9 (28) Asp9 Arg11/ Arg15 10 (Knappe et al. 2009 )

Caulosegnin I
Caulosegnin II
Caulosegnin III

19
19
19

1−
1−
2−

NMR
N.E./Char
N.E./Char

8 (26)
9 (29)
9 (29)

Glu8
Glu9
Glu9

Arg15/ Glu16
Tyr16
Arg15/ Tyr16

11
10
10

(Hegemann et al. 2013a )

Microcin J25 21 0 NMR 8 (26) Glu8 Phe19/ Tyr20 13 (Salomón and Farías 1992 ;

Bayro et al. 2003 ; Rosen-
gren et al. 2003 ; Wilson
et al. 2003 ; Rebuffat et al.
2004 )

Rhodanodin d 20/17 1−/0 N.E./Char 8 (26) Glu8 n.i. 12/9 (Hegemann et al. 2013b )

Rubrivinodin 18 1− N.E./Char 9 (29) Glu9 n.i. 9 (Hegemann et al. 2013b )

Sphingonodin I d
Sphingonodin II d

21/17
23/19

2−/4−
2−/0

N.E./Char
N.E./Char

8 (25)
8 (25)

Asp8
Asp8

n.i. 13/9

15/11

(Hegemann et al. 2013b )

Sphingopyxin I d 26/21

23/19

6−/4−
4−/2−

N.E./Char
N.E./Char

9 (28)
8 (25)

Asp9
Asp8

n.i. 17/12

15/11

(Hegemann et al. 2013b )