1. Hybrid MS instruments Tandem mass spectrometry, or MS/MS, has become the preferred technology for many applications in which mass spectrometry plays a part. First, tandem mass spectrometry allows selection and isolation of specific compounds of interest and their subsequent identification. Second, the extra selectivity of MS/MS enables this technology to be used for quantification […]
1. Introduction Identification of proteins by searching protein sequence collections with peptide mass fingerprinting data is widely used (Figure 1). The proteins in the sample are first separated to obtain one or a few proteins of interest. These proteins are then digested with a proteolytic enzyme. Mass spectra of the resulting peptide mixtures are acquired. […]