1. Introduction High-quality DNA is essential to obtaining the greatest success in DNA sequencing. Sequencing quality DNA should contain the lowest possible level of contaminating host DNA, have consistent yields from sample to sample, and be in sufficient quantities to perform multiple sequence reactions. Many thousands of clones are required whether a whole-genome shotgun or […]
1. Introductory remarks Reflecting on the past 15 years of the history of large-scale DNA sequencing, it is incredible to realize just how far this discipline has progressed in a relatively short period of time. The efforts, hopes, fears, and failures of many molecular biologists, engineers, and others have been played out in the arena […]