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(a)
Ex: 270
Tr yptophan
Raman
Gelbstoff
Porphyridium
Dunaliella
300
350
400
450
(b)
Ex: 420
Raman
Gelbstoff
Phycoerythrin
Chl a
Porphyridium
Dunaliella
450
500
550
600
650
700
(c)
Ex: 530
Phycoerythrin
Phycocyanin
Xantophyll
Porphyridium
Dunaliella
600
650
Wavelength (nm)
700
Figure 6.5 . Position of detection wavelengths collected using the submersible fluorometer designed
by GmbH.
(a)
ex 270 nm,
(b)
ex 420 nm, and
(c)
ex 530 nm. (Redrawn from Heuermann et al.,
1995
.).
developed by Sivaprakasam et al. in
2003
for both NOM and bisphenol-A detection. It
employed a 266 nm microchip laser and narrow bandwidth of filters (7.5-14 nm) resulting
in an improved sensitivity limit to 0.005 ppb QS, rivaling benchtop spectrofluorometers.
Shown in
Figure 6.6
are the 13 detection wavelengths overlaid on top of the emission spec-
trum of a discrete sample of NOM (
Figure 6.6A
) and changes in the NOM spectra for a
cruise tract from Tampa Bay, Florida out to Gulf of Mexico waters and returning to the bay
