Characterization of Bacillus Strains Producing Biosurfactants - Environmental Sustainability: Role of Green Technologies - page 173

Acknowledgement This work was conducted under the
project No. N N523 418237 from the Polish Ministry of
Science and Higher Education. This document was prepared
in conjunction with work accomplished under Contract
No. DE-AC09-08SR22470 with the US Department of
Energy.

towards supernatants obtained from the molasses
broth cultures. The inhibition was observed for
B. cinerea A 258, R. solani W 70, S. sclerotiorum
K 2291, Phomopsis diachenii K 657, C. dematium
K 425, P. complanata A 233, and P. exigua var.
exigua A 175. However, the supernatants obtained
from the brewery cultures inhibited mycelium
growth of Colletotrichum dematium K 425 and
Phoma species. The results obtained may be a
result of variable production of metabolites by
Bacillus strains because Gordillo et al. ( 2009 )
demonstrated that culture media composition
may also infl uence the production of metabolites
by Bacillus spp. Also, several studies have pro-
posed that LPs are co-produced and active in a
synergistic way, for example, surfactin with iturin,
surfactin with fengycin, and iturin with fengycin
(Ongena and Jacques 2008 ; Jacques 2011 ).

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8 Conclusions

The genus Bacillus includes many species with the
potential to produce LP biosurfactants of great
industrial, biotechnological, and environmental
interest. This is why many studies are conducted to
search for new biosurfactant-producing Bacillus
strains, their identifi cation, and the culture con-
ditions that can reduce LP production costs.
Bioconversion of industrial wastes into biosurfac-
tants has the potential to be a source of new materials
and can convert industrial waste into commercial
products. Our investigation confi rms that replacing
traditional microbiological media with agro-indus-
trial wastes as substrates for biosurfactant produc-
tion by three Bacillus has great potential. Moreover,
this will reduce many management problems
related to the processing of industrial waste. It has
been also shown that these three Bacillus spp.
grown on agro-industrial wastes, and their cell-
free supernatants as a source of biosurfactants,
had antifungal activities. Natural bacteria like
Bacillus , capable of suppressing pathogens and
maintaining their population by competing against
harmful microorganisms, could be successfully uti-
lized as biopesticides in agricultural biotechnology.
Finally, these Bacillus spp. grown on agro-industrial
wastes may have the potential for application as
biopesticides to control plant diseases.