Biomedical Engineering Reference
In-Depth Information
a
b
70
70
0.25µM Y6
+1.0µM YPFW
0.25µM Y6
60
60
50
50
40
40
30
30
20
20
10
10
0
0
0
5
10
15
20
25
30
0
5
10
15
20
25
30
Current (pA)
Current (pA)
c
d
70
70
0.25µM Y6
+1.0µM YPFW
+1.0µM YWPF
0.25µM Y6
60
60
60
+1.0µM YPFW
+1.0µM YWPF
+1.0µM YPWG
50
50
40
40
30
30
20
20
20
10
10
0
0
0
5
10
15
20
25
30
0
5
10
15
20
25
30
Current (pA)
Current (pA)
e
f
70
0.25µM Y6
60
+3.0µM YPFW
+1.0µM YWPF
+1.0µM YPWG
50
YPWG
YWPF
YPFW
40
30
20
10
Y6
0
0
5
10
15
20
25
30
Current (pA)
Fig. 13.2 Simultaneous detection of a mixture of peptides. Event histograms of (a) peptide Y6;
(b) a two-peptide mixture containing Y6 and YPFW; (c) a mixture of three peptides containing Y6,
YPFW and YWPF; (d) a mixture of four peptides containing Y6, YPFW, YWPF and YPWG; (e)a
mixture of Y6, YPFW, YWPF and YPWG, where the concentration of YPFW was three times
larger than that of (d); and (f) the corresponding representative single-channel current trace of (d),
in which the individual Y6, YPFW, YWPF and YPWG events were arrow-marked at different
levels. The experiments were performed at +30 mV (
cis
at ground) under a series of symmetrical
buffer conditions with a 2.0 mL solution comprising 2 M NaCl, and 10 mM Tris-HCl (pH 7.5).
a
HL protein (M113F)
7
was added to the
cis
compartment, while peptides Y6, YPFW, YWPF, and
YPWG were sequentially added to the
trans
compartment of the chamber with concentrations as
shown in the figure. Figure reprinted with permission from [
3
], Copyright 2009 American
Chemical Society
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