Biomedical Engineering Reference
In-Depth Information
Fig. 11.20 Current blockage
(
closed squares
) and
translocation time (
closed
triangles
) vs. viscosity for
3-kbp DNA in 1.5 M KCl-TE
solution at 120 mV bias
voltage. The
solid curves
are fits for
DI
t
1/
and
Dt
d
is the
viscosity of the buffer.
Image adapted from [
31
]
, where
Fig. 11.21 Current blockage
(
closed squares
) and
translocation time (
closed
triangles
) vs. applied voltage
measured in 1.6 M KCl-TE
buffer containing 20%
glycerol. The solid curves
are fits for
DI
b
V
and
t
d
1/V
. Image adapted
from [
31
]
Fig. 11.22 Current blockage
(
closed squares
) and
translocation time (
closed
triangles
) vs. applied voltage
change as a function of KCl
concentration. The
solid
curves
are linear fits of
current blockage and
translocation time to KCl
concentration. Image adapted
from [
31
]
concentration and decreasing the temperature. Note that these approaches also
decrease the ionic current signal by the same factor as that for the speed of DNA.
Thus the signal to noise ratio for DNA sequencing proposals based on the ionic
current measurement may degrade if using these methods for slowing down the
translocation speed of DNA.
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