Nanopore-Based DNA Sequencing and DNA Motion Control - Nanopores: Sensing and Fundamental Biological Interactions - page 270

Fig. 11.17 Principle of
Hybridization-assisted
Nanopore DNA Sequencing.
The upper panel shows a
single stranded DNA with
two DNA probes
translocating through a
nanopore. The lower panel
shows the corresponding
ionic current (not real data).
(Courtesy of X. Ling)

XXXXXXXXXXXXXXX
A

AGAT A AGAT

Fig. 11.18 Sequence
reconstruction from ionic
current traces. The upper
panel shows the ionic current
trace when the unknown
DNA with different DNA
probes translocates through
the nanopore. The lower
panel shows reconstructing
DNA sequence from the ionic
current trace. Three different
probes “TTAAG,” “AAGAT”
and “TTGTA” and their
corresponding ionic current
traces are shown in the figure.
(Courtesy of X. Ling)

A ATTCTAACATTCTA

AAGAT AAGAT

TTAAG

T TGTA

corresponding ionic current traces, then reconstructs the DNA sequencing from the
location information of the DNA probes. An example is shown in Fig. 11.18 : from
the ionic current trace of three different probes “TTAAG,” “AAGAT” and
“TTGTA,” one can reconstruct the original sequence of the target DNA “AATTC-
TAACATTCTA.” This proposal is currently under development by a company
called Nabsys ( http://www.nabsys.com/ ).