Capture and Translocation of Nucleic Acids into Sub-5 nm Solid-State Nanopores - Nanopores: Sensing and Fundamental Biological Interactions - page 246

Fig. 10.14 Plots of the
translocation timescale ( solid
circles ) for 400-bp DNA as a
function of nanopore
diameters ( d ) in the range
2.7-5 nm. The line is a guide
to the eye. Reproduced
with permission from
Wanunu et al. [ 49 ], Copyright
Elsevier Inc

Fig. 10.15 Log-log plot of DNA translocation timescales as a function of DNA length ( N )
measured using a 4-nm pore. Bi-exponential fits to the dwell-time distributions yield two
timescales: t 1 ( circles ) and t 2 ( squares ) which follow power laws with 2.28

respectively. The inset displays the dependence of the relative weight (the fraction of events in
the t 2 population vs. the t 1 population) as a function of DNA length. Reproduced with permission
from Wanunu et al. [ 49 ], copyright Elsevier Inc

a ¼ 1.40 and a ¼ 1 1

at the limits of both short and long DNA molecules, the dwell-time distributions are
mostly mono-exponential, with t 1 dominating the short molecules, and t 2 dominat-
ing for long molecules. Intermediate DNA lengths require the use of two exponen-
tials. Figure 10.15 displays a summary of characteristic translocation times for 11
different DNA lengths from ~150 to 20,000 bp, measured using a 4 nm pore at room
temperature with an applied bias of 300 mV [ 49 ]. For a DNA molecule shorter than