Biomedical Engineering Reference
In-Depth Information
Protein
Ligand
Pore
Parameter measured
10
9
M
Streptavidin-
functionalized
nanoparticles
Monoclonal anti-
streptavidin
antibody
Micropore in
PDMS
K
d
¼
3.5
0.5
W120A SA
a
0.01 s
1
Biotin
a
HL in lipid
bilayer
k
off
¼
0.31
10
6
M
1
s
1
K
d
¼
8.2
0.2
10
8
M
k
on
¼
3.8
0.3
k
off
¼
1.9
0.03
10
2
s
1
k
on
¼
4.9
1.0
10
7
M
1
s
1
K
d
¼
3.9
0.6
10
9
M
Monoclonal
anti-biotin IgG
Biotin
a
HL in lipid
bilayer
k
off1
¼
160
20 s
1
k
off2
¼
9.6
2.4 s
1
k
on
¼
1.0
0.4
10
9
M
1
s
1
K
d1
b
Gal-
b
-1,3-GalNAc
a
HL in lipid
bilayer
Lectin
10
3
M
¼
1.7
K
d12
b
10
4
M
¼
3.1
k
off
~1
5
s
1
k
on
¼
IgE
RNA Aptamer
Nanopore in
quartz pipette
10
5
M
1
s
1
1.9
10
9
M
K
d
~1
10
6
M
Carbonic
anhydrase II
Sulfonamide-
alamethicin
Alamethicin in
lipid bilayer
K
d
¼
1.9
0.9
a
W120A SA is a mutant form of streptavidin. This mutant has a reduced affinity for biotin, which
permitted the measurement of
k
off
.
b
K
d1
was the dissociation constant calculated for monovalent binding, and
K
d12
was the dissocia-
tion constant calculated assuming a monovalent step followed by a divalent step. See ref. [
21
] for
details.
Fig. 9.3 Detecting antibody binding to virus particles. Individual virus particles passing through a
conical nanopore (tip diameter of 650 nm) resulted in transient current pulses with a peak
amplitude proportional to the volume of the particle. Binding of antibodies to the virus particles
resulted in current pulses with an increased amplitude, indicating a larger volume for the
antibody-virus complex compared to virus particles in the absence of antibodies. Adopted from
Uram et al
.
with permission. Copyright Wiley-VCH Verlag GmbH & Co. KGaA [
50
]
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