Biomedical Engineering Reference
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Fig. 9.1 (a) General concept of the experimental setups used for chip-based nanopore sensing of
proteins and resulting current recording. A nanopore connects two electrolyte solutions that are
separated by the substrate in which the pore is fabricated. Often fluid channels on each side of the
substrate are created with poly(dimethylsiloxane) (PDMS). Application of an electric potential
difference results in ionic current through the pore. When a protein passes through the pore, the
current is reduced transiently. The magnitude,
DI
, the duration,
t
d
, and the frequency of these
current spikes are the primary signals used to determine information about proteins. (b) Concept
for experimental setups used in free-standing lipid bilayer-based nanopore sensing. In this
recording platform, a biological nanopore channel connects the two electrolyte solutions separated
by a planar lipid bilayer (BLM). Adopted from Macrae et al
.
with permission [
27
]
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