Membrane-Embedded Channel of Bacteriophage Phi29 DNA-Packaging Motor for Translocation and Sensing of Double-Stranded DNA - Nanopores: Sensing and Fundamental Biological Interactions - page 94

voltage (Fig. 4.10a , top). 141-bp DNA was added to the trans- side and samples
were taken from the cis- side for quantification at 30 min intervals. The number of
DNA molecules in the cis -chamber increased over time ( N ¼ 9 experiments)

and furthermore, DNA translocation rate was affected by the number of inserted
connectors. For comparison, control experiments were performed in the absence
of connectors (Fig. 4.10a , bottom) and in this case, the number of DNA molecules

Fig. 4.10 Quantitative PCR analysis of DNA translocation events. (a) Quantitative analysis of the
total number of DNA passing through one of the connectors in the lipid membrane from the trans -
chamber to the cis -chamber ( top ). Negative controls ( bottom ) were carried out under the same
condition but without connectors. The error bars represent standard deviations of the mean from
nine independent experiments and four negative control experiments. (b) A comparison of copy
number of translocated dsDNA measured by Q-PCR with the relevant blockade events counted
from current trace recorded in two independent experiments. The error bars represent the standard
deviation of three independent Q-PCR measurements. Figures reproduced with permissions from:
(a) Ref. [ 42 ], # Nature Publishing Group; (b) Ref. [ 43 ], # The Royal Society of Chemistry