Biomedical Engineering Reference
In-Depth Information
a
b
600
900
0.5 M NaCl (Slope 1.74)
1.0 M NaCl (Slope 3.05)
1.5 M NaCl (Slope 4.03)
2.0 M NaCl (Slope 4.64)
0.5 M KCl (Slope 2.90)
1.0 M KCl (Slope 4.86)
1.5 M KCl (Slope 6.33)
2.0 M KCl (Slope 7.72)
400
600
200
300
0
0
-300
-
200
-600
-
400
-900
-
600
-
100
-
50
0
50
100
-
100
-
50
0
50
100
Voltage (mV)
Voltage (mV)
c
d
25
0.5 M KCl
1.0 M KCl
1.5 M KCl
2.0 M KCl
0.5 M NaCl
1.0 M NaCl
1.5 M NaCl
2.0 M NaCl
40
20
30
15
20
10
10
5
0
0
1
2
3
4
5
1
2
3
4
5
N
umber of Inserted Connectors, N
Number of Inserted Connectors, N
e
f
200
140
180
120
160
140
100
120
80
100
60
80
60
40
40
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
2
3
4
5
6
7
8
Conductance of Single Connector, G
c
, NaCl/n
S
Conductance of Single Connector, G
c
, KCl/n
S
Fig. 4.6 Current-Voltage traces of single connector channel at different concentrations of NaCl
with 5 mM Tris, pH 7.8 (a) and different concentrations of KCl with 5 mM HEPES, pH 7.8 (b).
Relationship of measured conductance with number of connectors at different concentrations of
NaCl with 5 mM Tris, pH 7.8 (c) and different concentrations of KCl with 5 mM HEPES, pH 7.8
(d). All the conductance measurements were from more than three individual experiments. Error
bars represent standard deviations for the measurements. The regression equation for 0.5 M NaCl
is:
G
m
=nS ¼
1
:
73
N þ
0
:
01; for 1.0 M NaCl is:
G
m
=nS ¼
2
:
77
N þ
0
:
26; for 1.5 M NaCl is:
G
m
=nS ¼
3
:
81
N þ
0
:
26; and for 2.0 M NaCl is
G
m
=nS ¼
4
:
62
N
0
:
00. The regression
equation for 0.5 M KCl is:
G
m
=nS ¼
2
:
60
N þ
0
:
11; for 1.0 M KCl:
G
m
=nS ¼
4
:
73
N
0
:
10;
for 1.5 M KCl is:
G
m
=nS ¼
01,
respectively. Relationship of buffer conductivity versus conductance of single connector at 5 mM
Tris with 0.5-2.0 M NaCl (e) and at 5 mM HEPES with 0.5-2.0 M KCl (f). Figures reproduced
with permissions from: Ref. [
43
],
6
:
35
N
0
:
16; and for 2.0 M KCl is:
G
m
=nS ¼
7
:
86
N
0
:
The Royal Society of Chemistry
#
Search WWH ::
Custom Search