Biomedical Engineering Reference
In-Depth Information
Fig. 4.4 Conductance assays confirm the insertion of the connector into bilayer lipid membranes
(BLM). (a) BLMwith connector only (
top
) or liposomes only (
bottom
). (b) Addition of connector-
containing proteoliposomes resulted in multiple insertions. Inserts: Insertion of one (
top
) and two
connectors simultaneously (
bottom
). (c) One connector insertion at positive voltage (
top
) and at
negative voltage (
bottom
). Figures reproduced with permissions from: Ref. [
42
],
#
Nature
Publishing Group
a
b
1500
100
1 Pore (Slope 4.64)
2 Pores (Slope 9.31)
3 Pores (Slope 13.96)
1000
75
500
0
50
-
500
25
-
1000
-
1500
0
-
100
-
50
0
50
100
024681012
Voltage (mV)
Conductance (nS)
Fig. 4.5 (a) Data demonstrating robust properties of connector indicated by strong linear I-V
relationship. Increased number of connectors correlates to greater slopes but identical conductance
per channel. (b) Histogram showing uniform conductance of connector channels in the membrane.
Figures reproduced with permissions from: Ref. [
43
],
The Royal Society of Chemistry
#
nearly identical (Fig.
4.5b
), as demonstrated by a sharp Gaussian distribution.
For instance, the insertion of one connector in the BLM results in an increase in
the current of ~220 pA (equivalent to ~3 nS) at a potential of
75 mV in a buffer
composed of 5 mM Tris (pH 7.9)/1 M NaCl. Occasionally, simultaneous insertions
of two and three connectors can be observed in ~5% and 2% of all the connector
insertion events, respectively.
Search WWH ::
Custom Search