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of the migrating cells on a molecular level. The ability to introduce genes into
the migrating cells in the slice cultures will allow us to determine whether the
same processes that regulate migration in vitro also contribute to migration
in vivo.
The slice technology can be extrapolated to other systems in both
embryonic and adult tissues. Of particular interest will be studies observing
the molecular dynamics of migrating cells from transgenic mice with selected
genes deleted or mutated. Studies using knock-in mice with GFP-labelled
wild-type and mutant proteins will also be especially informative. Finally,
labelling the highly polarized cells that we observe in situ with antibodies
against specific phosphorylation activation states should provide insight into
which molecules are locally activated.
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