Biology Reference
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Figure 17.1 PH domain translocation regulated by PI3K and PTEN. (A) The PH domain
distributes uniformly in the cytosol before stimulation. After global stimulation with the
chemoattractant cAMP, PI3K phosphorylates the 3 position of the inositol ring of
PI(4,5)P
2
, producing PI(3,4,5)P
3
on the membrane. The PH domain binds to the PI(3,4,5)P
3
specifically and translocates to the membrane. PTEN dephosphorylates PI(3,4,5)P
3
and
produces PI(4,5)P
2
. (B) Cells were transformed with either the PhdA-GFP or the PI3K1-
GFP fusion construct. Translocation of PhdA and PI3K1 is shown in upper and lower
panels, respectively. Asterisks indicate the position of a micropipette filled with the
chemoattractant cAMP. As the chemoattractant source moves, PhdA and PI3K1 localize
to the new leading edge of the cell
