Biology Reference
In-Depth Information
filaments are important ways to induce actin assembly in response to signals.
However, in the past five years, we have gained an understanding of how the
WASp-Arp2/3 pathway, together with Ena/VASP proteins, can mediate actin
assembly in dynamic cell structures. Recent evidence indicates that there are
other ways to nucleate filament networks independently of the Arp2/3
complex, such as through the formins. As time progresses, we will hopefully
develop a deeper understanding of how the different systems for generating
actin filaments work together and thus a better idea of how the extremely
complex processes of cell movement and shape changes are regulated. No
doubt the next five years will also bring some new surprises and additional
players to the field - we can only look forward to a clearer, if more detailed,
picture of how actin dynamics allows cells to move and adapt to their
environment.
References
Alberts, A. S., 2001. Identification of a carboxyl-terminal diaphanous-related formin
homology protein autoregulatory domain. J. Biol. Chem. 276: 2824-2830.
Aspenstrom, P., Lindberg, U. and Hall, A., 1996. Two GTPases, Cdc42 and Rac, bind
directly to a protein implicated in the immunodeficiency disorder Wiskott-Aldrich
syndrome. Curr. Biol. 6: 70-75.
Bachmann, C., Fischer, L., Walter, U. and Reinhard, M., 1999. The EVH2 domain of the
vasodilator-stimulated phosphoprotein mediates tetramerization, F-actin binding, and
actin bundle formation. J. Biol. Chem. 274: 23 549-23 557.
Balasubramanian, M. K., Feoktistova, A., McCollum, D. and Gould, K. L., 1996. Fission
yeast Sop2p: a novel and evolutionarily conserved protein that interacts with Arp3p and
modulates profilin function. Embo J. 15: 6426-6437.
Bear, J. E., Loureiro, J. J., Libova, I., Fassler, R., et al., 2000. Negative regulation of
fibroblast motility by Ena/VASP proteins. Cell 101: 717-728.
Bear, J. E., Rawls, J. F. and Saxe, C. L. 3rd., 1998. SCAR, a WASP-related protein,
isolated as a suppressor of receptor defects in late Dictyostelium development. J. Cell
Biol. 142: 1325-1335.
Bear, J. E., Svitkina, T. M., Krause, M., Schafer, D. A., et al., 2002. Antagonism between
Ena/VASP proteins and actin filament capping regulates fibroblast motility. Cell 109:
509-521.
Ben-Yaacov, S., Le Borgne, R., Abramson, I., Schweisguth, F. and Schejter, E. D., 2001.
Wasp, the Drosophila Wiskott-Aldrich syndrome gene homologue, is required for cell
fate decisions mediated by Notch signaling. J. Cell Biol. 152: 1-13.
Benesch, S., Lommel, S., Steffen, A., Stradal, T. E., et al., 2002. Phosphatidylinositol 4,5-
biphosphate (PIP2)-induced vesicle movement depends on N-WASP and involves Nck,
WIP, and Grb2. J. Biol. Chem. 277: 37 771-37 776.
Carlier, M. F., Nioche, P., Broutin-L'Hermite, I., Boujemaa, R., et al., 2000. GRB2 links
signaling to actin assembly by enhancing interaction of neural Wiskott-Aldrich
syndrome protein (N-WASp) with actin-related protein (ARP2/3) complex. J. Biol.
Chem. 275: 21 946-21 952.
