Biology Reference
In-Depth Information
combination of variables would make the difference image very di
cult to
interpret.
We believe that FLAP and derivative methods will be extremely useful in
observing and analysing the complex spatio-temporal dynamics of the
cytoskeleton during such processes as cell adhesion, cell motility and cell
signalling.
Concluding remarks
The techniques based on bio-fluorescent probes presented here are being used
by an expanding number of scientists in biological research. The techniques
provide important information on dynamic localization with high sensitivity
covering steady state processes and specific protein-protein interactions. This
information complements advances in molecular genetics and biochemistry
and generates data essential for computer simulation models whichever
concept they may be based on: stochastic, systems biology, differential
equations or non-linear thermodynamics. These models are having an
increasing impact on our understanding of biological systems at different
levels: molecular, supra-molecular and cellular.
Acknowledgements
We would like to thank the following authors for allowing us to reproduce
some of their fine work (some of it unpublished): Christophe Ballestrem and
Benny Geiger (FRET), Natacha Edme, Simon Ameer-Beg, Boris Vojnovic
and Tony Ng (FLIM), Christien Merrifield and co-workers (TIRF), Gregory
Mashanov and Justin Molloy (TIRF), and Clare Waterman-Storer (FSM).
FLAP experiments were performed with the help of Colin Gray (Cancer
Research UK), Peter Jordan (Cancer Research UK) and Paul Fraylich
(MRC). Supported by a MRC programme grant (G.A.D.).
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