Biology Reference
In-Depth Information
Table 5.1
(Continued)
Purpose
Materials and Methods
Conclusion
Reference
Species and
genotype-specific
RAPD bands
in
Calamus
Daemonorops
and
Plectocomia
5 plants each in 15
different rattan genotypes,
13 belonging to the
genus
Calamus
and 1
each of Daemonorops
and Plectocomia, were
collected from Assam and
Arunachal Pradesh, India.
20 primers from OPD,
OPE, and OPL operon
series were tested; of these,
12 primers gave good
results.
Identified genotype-
specific markers suitable for
discriminating 7 genotypes of
Calamus
and
D. jenkinsianus
.
[12]
analyzing the diversity of populations of
Calamus metzianus
and
C. rivalis
[8]
and
C. thwaitesii
[9]
in South India and Sri Lanka, North East Himalaya, a megadiversity
region in India. DNA analysis indicated the populations of
C. thwaitesii
at Goa state
in India as having high genetic diversity
[9]
. Molecular markers helped to identify
the four diversity hot spot sites of the north-eastern Himalayas of India for conserv-
ing
C. flagellum
[10]
. ISSR marker analysis was attempted on
C. thwaitesii
popula-
tions in three types of locations (protected areas, buffer zones, and peripheral areas)
at three sites of Western ghats
[11]
. Protected areas conserve genetic resources of
rattans better than the other two types of locations, indicating the vulnerability of
rattans
[11]
. Species- and genotype-specific RAPD markers were discovered in
Calamus
[12]
and need conversion as SCAR markers and further validation.
5.2.3 Marker-Sex Association in Rattans
A male-specific RAPD
[13]
marker S1443 (5
-TCTCGCCTAC-3
), with an amplifica-
tion size of 500 bp in
Calamus simplicifolius
, was converted to a SCAR marker and
validated
[14]
. A female-specific ISSR marker (ISSR4—600 bp, (AAG)5CC-
[15]
was identified in
Calamus tenuis
.
5.3 Molecular Markers for Diversity and Sex Association in
Palmyra
Amplification of DNA of palmyra palms was attempted with 16 operon OPZ series
RAPD primers. Only seven primers (OPZ 01, OPZ 04, OPZ 05, OPZ 06, OPZ 07,
OPZ 10, and OPZ12) gave scorable bands and were used to analyze a set of 20
