Biology Reference
In-Depth Information
5.2.2 Molecular Markers for Diversity in Rattans
Six published coconut microsatellite markers
[6]
were tested for cross-amplification
in rattans
[7]
of four genera,
Korthalsia
,
Zalacca
,
Daemonorops
, and
Calamus
. Two
of these markers had shown amplification (
Table 5.1
). RAPD markers were used in
Table 5.1
Molecular Markers in Rattans
Purpose
Materials and Methods
Conclusion
Reference
Known SSR markers
in palms for rattans
CNZ05, CNZ19, CNZ34,
CNZ46, CN2A5, and
CN1G4 [6]
2 microsatellite primer pairs,
CNZ34 and CN2A5, for
molecular differentiation
of rattans. Lack of
polymorphism for CNZ34
and CN2A5 within
C.
thwaitesii
.
[7]
RAPD marker-aided
diversity in
C. metzianus
and
C. rivalis
in India and
Sri Lanka
65 operon RAPD primers
(OPA, OPB, OPE,
OPAU, OPAW series)
10 RAPD primers (OPAU02,
OPA04, OPA18, OPAW07,
OPAW09, OPAW10,
OPAW20, OPE02, OPE18,
and OPB15) good for
Calamus
;
C. metzianus
and
C. rivalis
clustered together.
Variability within populations
is less than the variability
between populations.
[8]
RAPD marker-
aided diversity in
C.
thwaitesii
in India and
Sri Lanka
80 individuals of 5
populations of Western
Ghats of India and 3
populations from Sri
Lanka; 100 operon
RAPD primers (OPA,
OPB, OPE, OPAU, and
OPAW)
10 RAPD primers (OPAU02,
OPA02, OPA11, OPAW07,
OPAW09, OPAW10,
OPAW20, OPE01,
OPE04, and OPB10) gave
scorable bands. Highest
genetic diversity measures
were observed in Goa
population. Variability within
populations: variability
is less than the variability
between populations; Sri
Lankan populations did not
discriminate from Indian
populations.
[9]
Locations type of
conservation on rattan
diversity using ISSR
markers
25 ISSR primers from the
UBC set (Sigma, USA)
were screened
10 primers (UBC 890, UBC
841, UBC 835, UBC 834,
UBC 868, UBC 880, UBC
855, UBC 848, ISSR 4, and
ISSR 5) gave good results.
[11]
