Biology Reference
In-Depth Information
Table 3.2
(Continued)
Markers
Germplasm or Somaclonal
Variation or Breeding
Conclusion
Reference
40 probes; 20 good
enough
54 palms in a breeding program
40 probes covering 60% of genome revealed relationship
from the study confirmed the known pedigree.
[59]
4 probes in 6 probe-
enzyme combination;
PoPgSP12 is highly
informative
probe.
111 elite breeding palms; 400
clonal lines
Identification of pedigree errors and proper crossing
strategy; elimination of individuals with recessive
deleterious genes; greater variation from unrelated
founder population.
[60]
SSR
5521 ESTs mined; 145
SSRs found in 136
unique ESTs. Primers
designed for 94;
primers for 10 EST-
SSRs were developed.
76 accessions of oil palm from
7 African countries
1
Deli
dura population
Genetic differentiation (FST) among the populations is
0.2492; high genetic diversity at Nigeria, Congo, and
Cameroon; some primers are transferable to coconut
and Jessenia bataua.
[61]
19,243 ESTs assembled to give
10,258 unique sequences; 629
ESTs were found to contain 722
SSRs.
Primer pairs were designed
for 405 unique EST-SSRs,
and 15 of these were used to
genotype 105
E. guineensis
and 30
E. oleifera
accessions.
14 SSRs were polymorphic.
Deli dura lost 45 alleles more than natural populations.
EST-SSR primer sEg00126 with (CGC)7 repeats a high
degree of sequence conservation across the palms.
[62]
