Biotechnology of Fruit Quality - Fruit Ripening: Physiology, Signalling and Genomics - page 282

Promoter
expression Transgene Metabolic reaction or function Metabolic phenotype Reference

Amino acid aromatic
decarboxylase ( AADC1A )
( Solanum lycopersicum )

Phenylalanine o phenylethylamine FMV 35S (OE) 10-fold n 1-nitro-2-phenylethane,

2-phenylethanol and
2-phenylacetaldehyde

Tieman et al. (2006b)

Salicylic acid methyltransferase
( SAMT ) ( S. lycopersicum )

Salicylic acid o methyl salicylate FMV 35S (OE) 123-fold n methyl salicylate Tieman et al. (2010)

Z -3 fatty acid desaturase ( FAD3 )
( Brassica napus ) or/and ( FAD7 )
( S. tuberosum )

Linoleic acid (18:2) o linolenic acid
(18:3)

CaMV 35S (OE) n 18:3/18:2 ratio Domínguez et al. (2010)

D -Zingiberene synthase ( ZIS )
( Ocimum basilicum )

Farnesyldiphosphate o
D -zingiberene

PG (OE) n D -zingiberene, other sesquiterpenes and

monoterpenes

Davidovich-Rikanati et
al. (2008)

Geraniol synthase ( GES ) ( O.
basilicum )

Geranyldiphosphate o geraniol PG (OE) n carotenoid-derived aroma volatiles; p

phytoene, lycopene and E -carotene

Davidovich-Rikanati et
al. (2007)

Carotenoid cleavage dioxygenase
( CCDIB ) ( S. lycopersicum )

Carotenoids o volatileterpenoid
compounds

FMV 35S (AS) 50% p E -ionone (50%); t 60% p

geranylacetone; no morphological
alterations or changes in carotenoids

Simkin et al. (2004)

Lipoxygenase ( TomLoxC ) ( S.
lycopersicum )

Chloroplast-targeted lipoxygenase
isoform, C6 volatiles made at the
expense of linoleic and linolenic
acids

CaMV 35S (AS) 1.5% p hexanal, hexenal and hexanol Chen et al. (2004)

S -linalool synthase ( LIS ) ( Clarkia
breweri )

Geranyldiphosphate o S -linalool E8 (OE) n S -Linalool and 8-hydroxylinalool; no

change in phenotype or in terpenoids

Lewinsohn et al. (2001)

Fibrillin ( FIB1 , FIB2 ) ( Capsicum
annuum )

Involved in synthesis of lipoproteins
in certain chromoplast types

Native (OE) 2-fold n carotenoids, i.e. 118% n lycopene;

64% n E -carotene, 36% n E -ionone, 74%
n E -cyclocitral, 50% n citral, 122% n
6-methyl-5-hepten-2-one and 223% n
geranylacetone

Simkin et al. (2007)

ODORANT 1 ( ODO1 ) ( Petunia
hybrida )

R2R3-type MYB transcription factor
that positively regulates volatile
benzoid levels, synthesizing
precursors from the shikimate
pathway

E8 (OE) No increase in phenylalanine-derived

volatile compounds

Dal Cin et al. (2011)

FMV, fi gwort mosaic virus. See Tables 16.1 and 16.2 for other abbreviations.