Environmental Engineering Reference
In-Depth Information
4. Concluding Remarks
ROS production and thus the potential danger of oxidative damage is an
inevitable consequence of life in oxygenic atmosphere. Plants have developed a
multifold system of protection against stress by UV-B (280-320 nm) irradiation, ranging
from morphological and metabolic changes to lessen radiation intensity inside the plant
through a system of ROS neutralising antioxidants to the repair of oxidatively damaged
molecules. ROS production and oxidative modification occur even under stress
conditions survived by plants, as a consequence of a balance between damage and
repair, ROS production and scavenging [46,47]. In this way, direct ROS monitoring is
an important, informative aspect of stress experiments, even under conditions when
certain physiological parameters do not seem affected. Nevertheless, direct ROS
trapping methods should be executed with care, being aware of potential pitfalls, such as
non ROS related conversion of the probe or its ROS adduct in the sample, localisation
and selectivity of the probe.
Acknowledgements
Research behind this overview was supported by grants from the Hungarian
National Research Foundation (OTKA T030362), the Hungarian Ministry of Education
(FKFP 0252/1999) and the Hungarian-Japanese Intergovernmental Science and
Technology Program (TéT JAP-8/989. Spin traps and ROS double sensors were
synthesised at the Department of Organic and Medicinal Chemistry, University of Pécs
by Prof. Kálmán Hideg, Drs. Tamás Kálai and Cecília P. Sár. Laser Scanning
Microscopy experiments were carried out at the Research Institute for Biological
Sciences (RIBS) Okayama (Japan) in collaboration with Dr. Ken'ichi Ogawa.
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